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Image Search Results
Journal: Diabetes
Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes
doi: 10.2337/db14-0929
Figure Lengend Snippet: Pancreas-infiltrating macrophages from superoxide-deficient NOD.Rag mice display a decrease in TNF-α and IL-1β synthesis, and a concomitant increase in Arg-1 synthesis after diabetogenic CD4 T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1– expressing (B) macrophages in the pancreata of NOD.Rag and NOD.Rag.Ncf1m1J recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 were calculated from CD11b+, I-Ag7+ cells. Data are representative of four independent experiments.
Article Snippet: CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the
Techniques: Expressing, Generated, Fluorescence
Journal: Diabetes
Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes
doi: 10.2337/db14-0929
Figure Lengend Snippet: Pancreas-infiltrating macrophages from NOD.Rag mice treated with MnP display a decrease in TNF-α and a concomitant increase in Arg-1 synthesis after diabetogenic CD4 T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1–expressing (B) macrophages in the pancreata of NOD.Rag recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells treated with HBSS or MnP. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 mean fluorescence intensity were calculated from CD11b+, I-Ag7+ cells. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells, while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Data are representative of four independent experiments.
Article Snippet: CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the
Techniques: Expressing, Generated, Fluorescence